Machine doesn't boot up when GPU is installed

I've recently updated BIOS to 1302 because GPU was running at x8 in the PCIEX16_1 slot After update BIOS, GPU-Z and CPU-Z all still show that it runs PCI-E

Is the PCIE connector damaged? Is the inside end of the graphics card hitting something else on the motherboard or in the case that prevents the

gpu with nvme slot How To Check PCIe Version of Slot and Motherboard PC Guide 101•63K How to Fix Stuck

gpu with m2 slot The Tweaker menu offers performance presets, adjusted thermal throttle limits, and overclocking for the CPU and GPU slot being PCIe